POLIMERAZA ZANJIR REAKSIYASI UCHUN REKOMBINANT VENT POLIMERAZA FERMENTINING ANALOGINI OLISH

U.A. Davlatboeva; I.I. Karimov; M.N. Naubetova; M.I. Muminov; Sh.N. Qo’ziyev; Sh.U. Turdikulova

doi:10.5281/

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Published: 2025-03-29

DOI: https://doi.org/10.5281/

Davlatboeva U.A.

Ilg’or texnologiyalar markazi, 100174 Toshkent, O’zbekiston O’zMU Biologiya va ekologiya fakulteti, 100174 Toshkent, O’zbekiston

Karimov I.I.

Ilg’or texnologiyalar markazi, 100174 Toshkent, O’zbekiston O’zMU Biologiya va ekologiya fakulteti, 100174 Toshkent, O’zbekiston

Naubetova M.N.

Ilg’or texnologiyalar markazi, 100174 Toshkent, O’zbekiston O’zMU Biologiya va ekologiya fakulteti, 100174 Toshkent, O’zbekiston

Muminov M.I.

Ilg’or texnologiyalar markazi, 100174 Toshkent, O’zbekiston O’zMU Biologiya va ekologiya fakulteti, 100174 Toshkent, O’zbekiston

Qo’ziyev Sh.N.

Ilg’or texnologiyalar markazi, 100174 Toshkent, O’zbekiston O’zMU Biologiya va ekologiya fakulteti, 100174 Toshkent, O’zbekiston

Turdikulova Sh.U.

Ilg’or texnologiyalar markazi, 100174 Toshkent, O’zbekiston O’zMU Biologiya va ekologiya fakulteti, 100174 Toshkent, O’zbekiston

Abstract

Molekulyar biologiya fanida polimerazalar asosiy fermentlar sifatida tan olingan bo‘lib, ular DNK va RNK sintezini amalga oshirishda muhim o‘rin egallaydi. DNK polimerazalari hujayralardagi matritsa DNK replikatsiyasi, ta'miri va rekombinatsiya jarayonlarini bevosita ishtirok etadi [1]. Ushbu fermentlarning biologik jarayonlardagi vazifalari qanchalik muhim bo‘lsa, ularning laboratoriya sharoitida DNK polimerazalarining yuqori haroratga chidamli turlari polimeraza zanjiri reaksiyasi (PZR) kabi texnologiyalarni amalga oshirishda katta ahamiyatga ega. Polimeraza zanjirli reaksiyasi DNK molekulalarini ko‘paytirish uchun keng qo‘llaniladigan texnologiya bo‘lib, u 1985-yilda Keri Mullis tomonidan ixtiro qilingan [2]. PZR yordamida atigi bir necha nusxada bo‘lgan DNK fragmentlari millionlab nusxaga ko‘paytirish mumkin.

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Issue

Vol. 1 No. 1 (2025): BIOTEXNOLOGIYANING RIVOJLANISH ISTIQBOLLARI VA MUAMMOLARI RЕSPUBLIKA ILMIY ANJUMANI, 28-29 mart 2025 y

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Articles

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Davlatboeva, U., Karimov, I., Naubetova, M., Muminov, M., Qo’ziyev, S., & Turdikulova, S. (2025). POLIMERAZA ZANJIR REAKSIYASI UCHUN REKOMBINANT VENT POLIMERAZA FERMENTINING ANALOGINI OLISH. Konferensiyalar, 1(1), 19-22. https://doi.org/10.5281/

References

Lehman, I. R., & Kaguni, L. S. (1989). DNA polymerase α. Journal of Biological Chemistry, 264(8), 4265–4268. https://doi.org/10.1016/S00219258(18)83733-4

Kornberg, A. (1988). DNA replication. Journal of Biological Chemistry, 263(1), 1–4. https://doi.org/10.1016/S0021-9258(19)57345-8 21

Mullis, K. B. (1990). The Unusual Origin of the Polymerase Chain Reaction. ScientificAmerican,262(4),56–65. https://doi.org/10.1038/scientificamerican049056

François Vigneault and Régen Drouin. 2005. Optimal conditions and specific characteristics of Vent exo– DNA polymerase in ligation-mediated polymerase chain reaction protocols. Biochemistry and Cell Biology. 83(2): 147165. https://doi.org/10.1139/o04-134